Recovery of dried samples from the Helminthological Collection of the Oswaldo Cruz Institute (CHIOC)/Fiocruz through specimen rehydration technique.

The Helminthological Collection of the Oswaldo Cruz Institute is the biggest in Latin America and it is among the largest collections at worldwide reference level, with around 40,000 sets of specimens and approximately one million individual specimens. It contains helminths parasites of vertebrate and invertebrate animals that form part of the fauna of Brazil and other countries. The samples comprise holotypes, paratypes and representative specimens of Platyhelminthes, Acanthocephala, Nematoda and other non-helminth phyla, such as Annelida and Arthropoda. Some of the samples preserved in liquid media were found to have dried out. This made it impossible to analyze these samples morphologically for taxonomic purposes. The aim of this study was to test techniques used for rehydration of the tegument of specimens that had been found to have dried out and present protocols for such techniques. A total of 528 specimens that either no longer were immersed in preservatives or had already dried out were analyzed: 96 digenetic trematodes, 45 cestodes, 22 acanthocephalans, 357 nematodes, four hirudineans and four pentastomid crustaceans. The technique of rehydration using only distilled water on the specimens proved to be efficient for recovering tegument malleability, for all samples analyzed in this present study.

Hemiurid and lecithasterid digenean trematodes and camallanid and cucullanid nematodes parasitizing flounders collected off the coast of Rio de Janeiro State, Brazil.

A total of 132 flounder specimens (60 Paralichthys isosceles, 36 Paralichthys patagonicus and 36 Xystreurys rasile) were collected off the coast of the state of Rio de Janeiro, Brazil. The fish were measured, necropsied, and had their organs investigated for hemiurid and lecithasterid digenean trematodes and camallanid and cucullanid nematodes. Taxonomic identification of the parasites was based on morphological and morphometric characters and was conducted using bright-field and scanning electron microscopies. The trematodes Lecithochirium monticellii and Aponurus laguncula were found parasitizing P. isosceles, P. patagonicus and X. rasile while the nematodes Procamallanus (Spirocamallanus) halitrophus and Cucullanus bonaerensis were found parasitizing P. isosceles and X. rasile and P. isosceles, P. patagonicus and X. rasile, respectively. Parasite indices of prevalence, mean intensity, mean abundance, and range of infection, as well as infection site, were evaluated for each parasite species. This study allowed to evidence the first occurrence of P. patagonicus by L. monticellii; X. rasile by A. laguncula and P. (S.) halitrophus; and P. isosceles and P. patagonicus by C. bonaerensis in the Western South Atlantic Ocean.

Hemiurid and lecithasterid digenean trematodes and camallanid and cucullanid nematodes parasitizing flounders collected off the coast of Rio de Janeiro State, Brazil / Trematodeos digeneticos hemiurideos e lecitasterideos e nematoides camalanideos e cuculanideos parasitando linguados coletados na costa do Estado do Rio de Janeiro, Brasil

Abstract A total of 132 flounder specimens (60 Paralichthys isosceles, 36 Paralichthys patagonicus and 36 Xystreurys rasile) were collected off the coast of the state of Rio de Janeiro, Brazil. The fish were measured, necropsied, and had their organs investigated for hemiurid and lecithasterid digenean trematodes and camallanid and cucullanid nematodes. Taxonomic identification of the parasites was based on morphological and morphometric characters and was conducted using bright-field and scanning electron microscopies. The trematodes Lecithochirium monticellii and Aponurus laguncula were found parasitizing P. isosceles, P. patagonicus and X. rasile while the nematodes Procamallanus (Spirocamallanus) halitrophus and Cucullanus bonaerensis were found parasitizing P. isosceles and X. rasile and P. isosceles, P. patagonicus and X. rasile, respectively. Parasite indices of prevalence, mean intensity, mean abundance, and range of infection, as well as infection site, were evaluated for each parasite species. This study allowed to evidence the first occurrence of P. patagonicus by L. monticellii; X. rasile by A. laguncula and P. (S.) halitrophus; and P. isosceles and P. patagonicus by C. bonaerensis in the Western South Atlantic Ocean.

Resumo Um total de 132 espécimes de linguados (60 Paralichthys isosceles, 36 Paralichthys patagonicus e 36 Xystreurys rasile) foram coletados na costa do Estado do Rio de Janeiro, Brasil. Os peixes foram medidos, necropsiados e tiveram seus órgãos investigados para o encontro de trematodeos digenéticos, das famílias Hemiuridae e Lecithasteridae, e nematoides das famílias Camallanidae e Cucullanidae. A identificação taxonômica dos parasitos foi baseada nos caracteres morfológicos e morfométricos, usando-se as microscopias de campo claro e eletrônica de varredura. Os trematódeos, Lecithochirium monticellii e Aponurus laguncula, foram encontrados parasitando P. isosceles, P. patagonicus e X. rasile, enquanto os nematoides, Procamallanus (Spirocamallanus) halitrophus e Cucullanus bonaerensis, foram encontrados parasitando P. isosceles e X. rasile e P. isosceles, P. patagonicus e X. rasile, respectivamente. Os índices parasitários de prevalência, intensidade média, abundância média e amplitude de variação da intensidade de infeção, bem como os sítios de infecção foram avaliados para cada espécie de parasito. Este é o primeiro registro de P. patagonicus por L. monticellii; X. rasile por A. laguncula e P. (S.) halitrophus; e de P. isosceles e P. patagonicus por C. bonaerensis no Oceano Atlântico Sul Ocidental.

Eustrongylides sp. (Nematoda, Dioctophymatoidea) parasitizing Hoplias malabaricus (Actinopterygii: Erythrinidae) collected from the state of Rio de Janeiro, Brazil / Eustrongylides sp. (Nematoda, Dioctophymatoidea) parasitando Hoplias malabaricus (Actinopterygii: Erythrinidae) coletados do Estado do Rio de Janeiro, Brasil

Abstract Hoplias malabaricus (Characiformes, Erythrinidae), trahira, is a neotropical freshwater fish of economic and public health significance. A total of 45 specimens of H. malabaricus commercialized in the municipality of Magé, state of Rio de Janeiro, Brazil, were acquired between April 2016 and April 2018 to investigate the presence of nematode larvae. Twenty of the fish were found parasitized by 347 fourth-stage nematode larvae identified taxonomically as Eustrongylides sp. using morphological, morphometric and molecular data. The parasitic indices were: prevalence 44.44%, mean intensity 17.35, mean abundance 7.71, and range of infection 2-40. Infection sites were musculature, mesentery, abdominal cavity, and serosa of intestine, stomach and liver. This is the first report of Eustrongylides sp. larvae parasitizing H. malabaricus in the state of Rio de Janeiro.

Resumo Hoplias malabaricus (Characiformes, Erythrinidae), traíra, é um peixe neotropical de água doce que tem significante impacto na economia e saúde pública. De abril de 2016 a abril de 2018, foram adquiridos 45 espécimes de H. malabaricus comercializados no município de Magé, Estado do Rio de Janeiro, Brasil. Os peixes foram necropsiados e filetados para investigação da presença de larvas de nematoides. Vinte dos peixes coletados estavam parasitados por 347 larvas de nematoides, identificadas taxonomicamente como larvas de quarto estágio de Eustrongylides sp. usando-se dados morfológicos, morfométricos e moleculares, apresentando os seguintes valores: prevalência de 44,44%, intensidade média de 17,35, abundância média de 7,71, e amplitude de variação da infecção de 2-40. Os sítios de infecção foram musculatura, mesentério, cavidade abdominal e serosas do intestino, estômago e fígado. Este é o primeiro registro de larvas de Eustrongylides sp. parasitando H. malabaricus no Estado do Rio de Janeiro.

Trypanorhynch cestodes parasitizing Mugil liza (Mugiliformes: Mugilidae) commercialized in the state of Rio de Janeiro, Brazil / Cestoides Trypanorhyncha parasitando Mugil liza (Mugiliformes: Mugilidae) comercializados no estado do Rio de Janeiro, Brasil

Abstract The aim of the present study was to identify, through morphological and morphometric analyses, the species of trypanorhynch cestodes found as plerocerci in the intestinal serosa of Mugil liza and to determine their parasitic indices. One hundred and fifty specimens of this mullet collected off the coast of the state of Rio de Janeiro were necropsied and the trypanorhynch cestodes found were fixed and preserved as whole mounts for morphological analysis. The plerocerci were identified as Callitetrarhynchus gracilis and Pterobothrium crassicole, both with a prevalence of 0.67%, an intensity of 1 and abundance of 0.0067, in single infections. This is the first record of a trypanorhynch cestode parasitizing M. liza in Brazil. Although the parasitic indexes were low, from a hygienic-sanitary perspective the plerocerci of these species are visible to the naked eye, and thus can disgust consumers and make marketing the fish unfeasible.

Resumo O presente estudo objetivou determinar taxonomicamente as espécies de plerocercos de cestoides Trypanorhyncha encontradas na serosa intestinal das tainhas Mugil liza, através do estudo morfológico e morfométrico e indicar seus índices parasitários. Foram coletados 150 espécimes desta espécie de tainha do litoral do estado do Rio de Janeiro. Após as necropsias, os cestoides Trypanorhyncha encontrados foram fixados e preservados em montagens permanentes para permitir a análise de suas estruturas morfológicas. Os plerocercos foram identificados como Callitetrarhynchus gracilis e Pterobothrium crassicole e ambas as espécies apresentaram prevalência de 0,67%, intensidade de 1 e abundância de 0,0067, em infecções únicas. Este é o primeiro registro de cestoides Trypanorhyncha parasitando M. liza no Brasil. Quanto ao aspecto higiênico-sanitário, vale ressaltar que, embora os índices parasitários registrados tenham sido baixos, os plerocercos dessas espécies estavam visíveis a olho nu, podendo causar repugnância ao consumidor e inviabilizar a comercialização do pescado.

Eustrongylides sp. (Nematoda, Dioctophymatoidea) parasitizing Hoplias malabaricus (Actinopterygii: Erythrinidae) collected from the state of Rio de Janeiro, Brazil.

Hoplias malabaricus (Characiformes, Erythrinidae), trahira, is a neotropical freshwater fish of economic and public health significance. A total of 45 specimens of H. malabaricus commercialized in the municipality of Magé, state of Rio de Janeiro, Brazil, were acquired between April 2016 and April 2018 to investigate the presence of nematode larvae. Twenty of the fish were found parasitized by 347 fourth-stage nematode larvae identified taxonomically as Eustrongylides sp. using morphological, morphometric and molecular data. The parasitic indices were: prevalence 44.44%, mean intensity 17.35, mean abundance 7.71, and range of infection 2-40. Infection sites were musculature, mesentery, abdominal cavity, and serosa of intestine, stomach and liver. This is the first report of Eustrongylides sp. larvae parasitizing H. malabaricus in the state of Rio de Janeiro.

Trypanorhynch cestodes parasitizing Mugil liza (Mugiliformes: Mugilidae) commercialized in the state of Rio de Janeiro, Brazil.

The aim of the present study was to identify, through morphological and morphometric analyses, the species of trypanorhynch cestodes found as plerocerci in the intestinal serosa of Mugil liza and to determine their parasitic indices. One hundred and fifty specimens of this mullet collected off the coast of the state of Rio de Janeiro were necropsied and the trypanorhynch cestodes found were fixed and preserved as whole mounts for morphological analysis. The plerocerci were identified as Callitetrarhynchus gracilis and Pterobothrium crassicole, both with a prevalence of 0.67%, an intensity of 1 and abundance of 0.0067, in single infections. This is the first record of a trypanorhynch cestode parasitizing M. liza in Brazil. Although the parasitic indexes were low, from a hygienic-sanitary perspective the plerocerci of these species are visible to the naked eye, and thus can disgust consumers and make marketing the fish unfeasible.

Acanthocephalan parasites of the flounder species Paralichthys isosceles, Paralichthys patagonicus and Xystreurys rasile from Brazil / Acantocéfalos parasitos de espécies de linguados Paralichthys isosceles, Paralichthys patagonicus e Xystreurys rasile no Brasil

Abstract Flounders are commercially and economically important fish. A total of 120 specimens of flounders (60 Paralichthys isosceles, 30 Paralichthys patagonicus and 30 Xystreurys rasile) were collected off the coast of the state of Rio de Janeiro, Brazil. The fish were measured, necropsied and filleted, and then had their organs investigated for acanthocephalans. Taxonomic identification of the parasites was based on morphological, morphometric and genetic characters. Paralichthys isosceles and P. patagonicus were parasitized by juveniles of Serrasentis sagittifer, Bolbosoma turbinella, Corynosoma australe and C. cetaceum; Xystreurys rasile was parasitized by C. australe. Genetic characterization confirmed the identification of specimens of Bolbosoma turbinella and Corynosoma australe, as demonstrated by phylogenetic analyses using both ITS and cox1 molecular targets. Parasite indices of prevalence, intensity, mean intensity, abundance, mean abundance, and range of infection, as well as infection site, were evaluated for each parasite species. This is the first report of S. sagittifer parasitizing P. isosceles and P. patagonicus, and B. turbinella parasitizing P. patagonicus.

Resumo Os linguados são peixes comercial e economicamente importantes. Um total de 120 espécimes de linguados (60 Paralichthys isosceles, 30 P. patagonicus e 30 Xystreurys rasile) foram coletados no litoral do estado do Rio de Janeiro, Brasil. Os peixes foram medidos, necropsiados, filetados e tiveram seus órgãos investigados para a presença de acantocéfalos. A identificação taxonômica foi baseada em caracteres morfológicos, morfométricos e genéticos. Paralichthys isosceles e P. patagonicus estavam parasitados por acantocéfalos juvenis de Serrasentis sagittifer, Bolbosoma turbinella, Corynosoma australe e C. cetaceum; Xystreurys rasile estava parasitado com C. australe. A caracterização genética confirmou a identificação dos espécimes de Bolbosoma turbinella e Corynosoma australe, como demonstrado por análises filogenéticas usando ambos marcadores moleculares ITS e cox1. Foram analisados os índices parasitários: prevalência, intensidade, intensidade média, abundância, abundância média, amplitude de variação da infecção e sítio de infecção de cada espécie de parasito. Este é o primeiro registro de S. sagittifer parasitando P. isosceles e P. patagonicus, e de B. turbinella parasitando P. patagonicus.

Acanthocephalan parasites of the flounder species Paralichthys isosceles, Paralichthys patagonicus and Xystreurys rasile from Brazil.

Flounders are commercially and economically important fish. A total of 120 specimens of flounders (60 Paralichthys isosceles, 30 Paralichthys patagonicus and 30 Xystreurys rasile) were collected off the coast of the state of Rio de Janeiro, Brazil. The fish were measured, necropsied and filleted, and then had their organs investigated for acanthocephalans. Taxonomic identification of the parasites was based on morphological, morphometric and genetic characters. Paralichthys isosceles and P. patagonicus were parasitized by juveniles of Serrasentis sagittifer, Bolbosoma turbinella, Corynosoma australe and C. cetaceum; Xystreurys rasile was parasitized by C. australe. Genetic characterization confirmed the identification of specimens of Bolbosoma turbinella and Corynosoma australe, as demonstrated by phylogenetic analyses using both ITS and cox1 molecular targets. Parasite indices of prevalence, intensity, mean intensity, abundance, mean abundance, and range of infection, as well as infection site, were evaluated for each parasite species. This is the first report of S. sagittifer parasitizing P. isosceles and P. patagonicus, and B. turbinella parasitizing P. patagonicus.

Estudo preliminar de Hysterothylacium deardorffoverstreetorum (Nematoda, Raphidascarididae) parasitando pargo, Pagrus pagrus, coletados no litoral do Estado do Rio de Janeiro, RJ, Brasil / Preliminary study of Hysterothylacium deardorffoverstreetorum (Nematoda, Raphidascarididae) parasitizing red porgy, Pagrus pagrus, collected in the coast of the State of Rio de Janeiro, RJ, Brazil

O pargo, Pagrus pagrus, possui carne muito apreciada na culinária brasileira. Peixes marinhos podem albergar larvas de nematoides anisaquídeos, que podem causar anisaquidose no ser humano, se ingerirem peixes crus ou mal cozidos. Entre os meses de fevereiro e julho de 2017, foram coletados 115 peixes em mercados de pescados no Município de Arraial do Cabo, RJ. Os peixes foram necropsiados e 17 estavam parasitados por anisaquídeos. Os sítios de infecção foram intestino, fígado e cavidade abdominal. Os nematoides foram processados seguindo as técnicas helmintológicas e identificados em taxonomicamente como Hysterothylacium deardorffoverstreetorum. O objetivo foi identificar a nível específico os nematoides coletados das lesões das serosas de P. pagrus e relatar alterações patológicas encontradas.

Pterobothrium sp. parasitando linguado, Xystreurys rasile (Jordan, 1891), coletados no litoral do Estado do Rio de Janeiro, Brasil e sua importância na inspeção do pescado / Pterobothrium sp. parasitizing flounders, Xystreurys rasile (Jordan, 1891), collected off the coast the State of Rio de Janeiro, Brazil and its fishing inspection importance

Alguns parasitos, podem conferir aspecto repugnante ao pescado, sendo condenados pelo Serviço de Inspeção, quando na indústria de beneficiamento. Entre novembro de 2010 e maio de 2018 foram adquiridos 36 espécimes de Xystreurys rasile em mercados de pescados no município do Rio de Janeiro, Brasil. Os peixes foram identificados, medidos, pesados e necropsiados. Os cestoides foram processados segundo as técnicas usuais em helmintologia. Cinco linguados estavam parasitados por 29 cestoides do gênero Pterobothrium, os índices parasitários foram: prevalência (14%), intensidade média (5,8), abundância média (0,8) e amplitude de infecção (2-12) e os sítios de infecção foram estômago, intestino, rim, fígado, serosas do estômago, baço e fígado e cavidade abdominal. Este é o primeiro registro de Pterobothrium sp. parasitando X. rasile.

Tentacularia coryphaenae (Cestoda: Trypanorhyncha) em Fistularia petimba Lacépède, 1803 coletados Estado do Rio de Janeiro, Brasil e sua importância na inspeção do pescado / Tentacularia coryphaenae (Cestoda: Trypanorhyncha), Fistularia petimba Lacépède, 1803 collected off the coast of the State of Rio de Janeiro, Brazil and its importance in fish inspection

A presença de helmintos no pescado resulta em perdas econômicas devido a repugnância, além do risco de desenvolver reações alérgicas, reforçando a importância da inspeção higiênico-sanitária, como o caso dos cestoides da ordem Trypanorhyncha. Entre janeiro e setembro de 2018 foram adquiridos 30 espécimes de Fistularia petimba no Estado do Rio de Janeiro. Após a necropsia no Laboratório de Inspeção e Tecnologia de Pescado da Faculdade de Veterinária-UFF, foi verificado a positividade de dois peixes para o cestoide identificado como sendo da espécie Tentacularia coryphaenae, presente na cavidade abdominal e mesentério, apresentando os seguintes índices parasitários: prevalência 6,6%, intensidade média 1; abundância média 0,06.

Atractis thapari (Nematoda, Atractidae) parasitizing Chelonoidis carbonarius and C. denticulatus (Testudinidae) in the state of Piauí, Brazil / Atractis thapari (Nematoda, Atractidae) parasitando Chelonoidis carbonarius e C. denticulatus (Testudinidae) no estado do Piauí, Brasil

Abstract Atractis thapari Petter, 1966, an atractid nematode, was collected parasitizing the large intestine of tortoises of the species Chelonoidis carbonarius (Spix, 1824) (Cc) and C. denticulatus (Linnaeus, 1766) (Cd) (Testudinidae) in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. Taxonomic identification was based on morphological and morphometrical features, and using bright-field and scanning electron microscopy. The present study adds new observations on the morphology of A. thapari, mainly relating to mouth papillae, the excretory pore, deirids, and male and female posterior ends. The parasitic indices of prevalence (P), mean intensity (MI), mean abundance (MA) and range of infection (RI) for A. thapari in these two tortoise species were: P = 100%, MI = 154,667, MA = 154,667, RI = 5,500-588,500 (Cc); P = 100%, MI = 93,639, MA = 93,639, RI = 1,000-224,500 (Cd). This report confirms the occurrence of A. thapari in Neotropical region, South America, Brazil, and extends its occurrences to a new host, the tortoise C. carbonarius. Adjustment of host management with the aim of improving hygiene and health conditions is suggested.

Resumo Atractis thapari Petter, 1966, um nematoide atractídeo, foi coletado parasitando o intestino grosso de jabutis, Chelonoidis carbonarius (Spix, 1824) (Cc) e C. denticulatus (Linnaeus, 1766) (Cd) no Parque Zoobotânico, município de Teresina, estado do Piauí, Brasil. A identificação taxonômica foi baseada nos caracteres morfológicos e morfométricos usando microscopias de campo claro e eletrônica de varredura. O presente estudo adiciona novas observações na morfologia, principalmente relacionadas as papilas bucais, poro excretor, deirídeos, e extremidade posterior de machos e fêmeas. Os índices parasitários de prevalência (P), intensidade média (IM), abundância média (AM) e amplitude de variação de infecção (AI) de A. thapari em ambos jabutis foram P = 100%, IM = 154.667, AM = 154.667, AI = 5.500-588.500 (Cc); P = 100%, I M= 93.639, AM = 93.639, AI = 1.000-224.500 (Cd). Este registro confirma a ocorrência de A. thapari na região Neotropical, América do Sul, Brasil, e expande a um novo hospedeiro, o jabuti C. carbonarius. É sugerido um ajuste no manejo dos hospedeiros objetivando melhora das suas condições higiênico sanitárias.

Atractis thapari (Nematoda, Atractidae) parasitizing Chelonoidis carbonarius and C. denticulatus (Testudinidae) in the state of Piauí, Brazil.

Atractis thapari Petter, 1966, an atractid nematode, was collected parasitizing the large intestine of tortoises of the species Chelonoidis carbonarius (Spix, 1824) (Cc) and C. denticulatus (Linnaeus, 1766) (Cd) (Testudinidae) in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. Taxonomic identification was based on morphological and morphometrical features, and using bright-field and scanning electron microscopy. The present study adds new observations on the morphology of A. thapari, mainly relating to mouth papillae, the excretory pore, deirids, and male and female posterior ends. The parasitic indices of prevalence (P), mean intensity (MI), mean abundance (MA) and range of infection (RI) for A. thapari in these two tortoise species were: P = 100%, MI = 154,667, MA = 154,667, RI = 5,500-588,500 (Cc); P = 100%, MI = 93,639, MA = 93,639, RI = 1,000-224,500 (Cd). This report confirms the occurrence of A. thapari in Neotropical region, South America, Brazil, and extends its occurrences to a new host, the tortoise C. carbonarius. Adjustment of host management with the aim of improving hygiene and health conditions is suggested.

Type material of Acanthocephala, Nematoda and other non-helminths phyla (Cnidaria, Annelida, and Arthropoda) housed in the Helminthological Collection of the Oswaldo Cruz Institute/ FIOCRUZ (CHIOC), Rio de Janeiro, Brazil, from 1979 to 2016.

The third part of the catalogue of type material in the Helminthological Collection of the Oswaldo Cruz Institute/FIOCRUZ (CHIOC), comprising types deposited between 1979 and 2016, is presented to complement the first list of all types that was published in 1979. This part encompasses Acanthocephala, Nematoda and the other non-helminth phyla Cnidaria, Annelida, and Arthropoda. Platyhelminthes was covered in the first (Monogenoidea) and second (Rhabditophora Trematoda and Cestoda) parts of the catalogue published in September 2016 and March 2017, respectively. The present catalogue comprises type material for 116 species distributed across five phyla, nine classes, 50 families, and 80 genera. Specific names are listed systematically, followed by type host, infection site, type locality, and specimens with their collection numbers and references. Species classification and nomenclature are updated.

Chapiniella variabilis (Nematoda) parasitizing Chelonoidis carbonarius and C. denticulatus (Testudinidae) in the state of Piauí / Chapiniella variabilis (Nematoda) parasitando Chelonoidis carbonarius e C. denticulatus (Testudinidae) do estado do Piauí

Abstract Chapiniella variabilis (Chapin, 1924), a strongylid nematode, was collected parasitizing the large intestine of the tortoises Chelonoidis carbonarius (Spix, 1824) (Cc) and C. denticulatus (Linnaeus, 1766) (Cd) in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. The taxonomic identification was based on morphological and morphometric features, using bright-field and scanning electron microscopy. The present study adds new observations on the morphology, mainly relating to the mouth papillae, external and internal leaf-crown elements, excretory pore, deirids and male and female posterior end. The parasitic indices of prevalence (P), mean intensity (MI), mean abundance (MA) and range of infection (RI) of C. variabilis in these two tortoise species were: P = 100%, MI = 833.3, MA = 833.3, RI = 500-1,500 (Cc); P = 100%, MI = 472.2, MA = 472.2, RI = 333-500 (Cd). This record expands occurrences of C. variabilis to a new host, C. carbonarius, and to another state in Brazil, in the Neotropical region of South America. Adjustment to host management with the aim of improving hygiene and health conditions is suggested.

Resumo Chapiniella variabilis (Chapin, 1924), um nematoide estrongilídeo, foi coletado parasitando o intestino grosso de jabutis, Chelonoidis carbonarius (Spix, 1824) e C. denticulatus (Linnaeus, 1766) do Parque Zoobotânico, município de Teresina, estado do Piauí, Brasil. A identificação taxonômica foi baseada nos caracteres morfológicos e morfométricos usando microscopias de campo claro e eletrônica de varredura. O presente estudo adiciona novas observações na morfologia, principalmente relacionadas as papilas bucais, elementos externos e internos da coroa-foliar, poro excretor, deirídeos, e extremidade posterior de machos e fêmeas. Os índices parasitários de prevalência (P), intensidade média (IM), abundância média (AM) e amplitude de variação de infecção (AI) de C. variabilis em ambos jabutis foram P = 100%, IM = 833,3, AM = 833,3, AI = 500-1.500 (Cc); P = 100%, IM = 472,2, AM = 472,2, AI = 333-500 (Cd). Este registro aumenta a ocorrência de C. variabilis a um novo hospedeiro, C. carbonarius, e a um outro estado do Brasil, na região Neotropical da América do Sul. É sugerido um ajuste no manejo dos hospedeiros objetivando melhora das suas condições higiênico sanitárias.

Type material of Platyhelminthes housed in the Helminthological Collection of the Oswaldo Cruz Institute/ FIOCRUZ (CHIOC), Rio de Janeiro, Brazil, from 1979 to 2016 (Rhabditophora, Trematoda and Cestoda).

The second part of the catalogue of type material deposited in the Helminthological Collection of the Oswaldo Cruz Institute/ FIOCRUZ (CHIOC), between 1979 and 2016, is presented, to complement the first list of all types that was published in 1979. This part includes Platyhelminthes other than monogenoids, which were covered in the first part published in September 2016. The present catalogue comprises type material for 104 species, distributed across three classes, 40 families and 75 genera. Species names are listed systematically, followed by type host, infection site, type locality and specimens with their collection numbers and references. The classification and the nomenclature of the species have been updated.

Histopathological changes induced by Hysterothylacium deardorffoverstreetorum larvae (Nematoda: Raphidascarididae) in Priacanthus arenatus Cuvier, 1829 (Actinopterygii).

Priacanthus arenatus is a fish that occurs in the waters of the western Atlantic Ocean and has few records of parasitism. This study aimed to report histological changes caused by the larvae of Hysterothylacium deardorffoverstreetorum. Between July and December, 2013, 30 samples of P. arenatus were obtained from fish markets in the cities of Niterói and Rio de Janeiro, state of Rio de Janeiro, Brazil. Fragments of spleen, liver, stomach wall and mesentery tissues containing nodules and free parasites were extracted to histopathological study. Through histological analysis it was verified that these nodules consisted of connective tissue capsules, in which the parasites were located, with granulomatous inflammation mainly comprised of macrophages and lymphocytes. These nodules also consisted of a central portion of acellular amorphous material, formed by concentric layers, which was slightly basophilic and contained tunnels and, sometimes, parasite debris. The presence of tunnels in acellular amorphous material suggests that the larvae are initially located at the center of the nodules and then they migrate to the surface.

Histopathological changes induced by Hysterothylacium deardorffoverstreetorum larvae (Nematoda: Raphidascarididae) in Priacanthus arenatus Cuvier, 1829 (Actinopterygii) / Alterações histopatologicas induzidas por larvas de Hysterothylacium deardorffoverstreetorum (Nematoda: Raphidascarididae) em Priacanthus arenatus Cuvier, 1829 (Actinopterygii)

Abstract Priacanthus arenatus is a fish that occurs in the waters of the western Atlantic Ocean and has few records of parasitism. This study aimed to report histological changes caused by the larvae of Hysterothylacium deardorffoverstreetorum. Between July and December, 2013, 30 samples of P. arenatus were obtained from fish markets in the cities of Niterói and Rio de Janeiro, state of Rio de Janeiro, Brazil. Fragments of spleen, liver, stomach wall and mesentery tissues containing nodules and free parasites were extracted to histopathological study. Through histological analysis it was verified that these nodules consisted of connective tissue capsules, in which the parasites were located, with granulomatous inflammation mainly comprised of macrophages and lymphocytes. These nodules also consisted of a central portion of acellular amorphous material, formed by concentric layers, which was slightly basophilic and contained tunnels and, sometimes, parasite debris. The presence of tunnels in acellular amorphous material suggests that the larvae are initially located at the center of the nodules and then they migrate to the surface.

Resumo Priacanthus arenatus é um peixe que ocorre nas águas do Atlântico ocidental com poucos relatos de parasitimo. Este estudo objetivou relatar as características histológicas causadas pelas larvas de H. deardorffoverstreetorum em P. arenatus. Entre julho e dezembro de 2013, 30 espécimes de P. arenatus foram obtidas de mercados de peixes das cidades de Niterói e Rio de Janeiro, Estado do Rio de Janeiro, Brasil. Fragmentos de baço, fígado, parede do estômago e mesentério contendo nódulos foram extraídos para estudo histopatológico. Foi observado através de análise histológica que esses nódulos eram constituídos por uma cápsula de tecido conjuntivo, onde se localizavam os parasitas viáveis, com infiltrado inflamatório granulomatoso, constituído, predominantemente, por macrófagos e linfócitos e por porção central de material amorfo acelular, formado por lâminas concêntricas, ligeiramente basofílico, contendo túneis e, por vezes, restos parasitários. A presença de túneis no material amorfo acelular dos nódulos analisados histologicamente sugerem que as larvas se localizam inicialmente no centro do granuloma e depois migram para a superfície do mesmo.

Chapiniella variabilis (Nematoda) parasitizing Chelonoidis carbonarius and C. denticulatus (Testudinidae) in the state of Piauí.

Chapiniella variabilis (Chapin, 1924), a strongylid nematode, was collected parasitizing the large intestine of the tortoises Chelonoidis carbonarius (Spix, 1824) (Cc) and C. denticulatus (Linnaeus, 1766) (Cd) in the Zoobotanical Park of the municipality of Teresina, state of Piauí, Brazil. The taxonomic identification was based on morphological and morphometric features, using bright-field and scanning electron microscopy. The present study adds new observations on the morphology, mainly relating to the mouth papillae, external and internal leaf-crown elements, excretory pore, deirids and male and female posterior end. The parasitic indices of prevalence (P), mean intensity (MI), mean abundance (MA) and range of infection (RI) of C. variabilis in these two tortoise species were: P = 100%, MI = 833.3, MA = 833.3, RI = 500-1,500 (Cc); P = 100%, MI = 472.2, MA = 472.2, RI = 333-500 (Cd). This record expands occurrences of C. variabilis to a new host, C. carbonarius, and to another state in Brazil, in the Neotropical region of South America. Adjustment to host management with the aim of improving hygiene and health conditions is suggested.